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A primary concern of the blood collector for this test is preventing contamination of the blood culture by skin organisms.
Procedure:
Do not touch the venipuncture site after it has been prepared. Do not put unsterile gauze over venipuncture site until after blood is drawn.
Apply the tourniquet to the patient's arm and select a site for the venipuncture. Remove the tourniquet.
To prepare the patient's arm, cleanse the site with a betadine swab in a circular motion beginning at the center of the site selected for venipuncture. Allow the betadine solution to air dry.
Cleanse the tops of blood culture collection bottles with betadine.
Reapply the tourniquet.
Obtain blood with a 10 ml vaccutainer and a 22 gauge or 20 gauge needle, pushing out all the air from the vaccutainer before drawing blood.
Change needles and inject the blood into the tops of the previously prepared anaerobic (yellow) x1 and aerobic (blue) blood culture bottles x1 and denote whether obtained from (R) Right or Left (L) arm.
Invert the bottles gently and label the usual patient name, date, and time of collection.
After the patient's arm has stopped bleeding remove the betadine solution by wiping with a clean alcohol prep.
Indicate on the requisition and on the bottles which culture (number) you have drawn if a series of blood cultures have been ordered.
Blood cultures may not be ordered any less than one hour apart. If the doctor desires cultures more often, he must consult the supervisor of Microbiology of the testing laboratory.
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